Composite
Propane1

Part:BBa_K1655000:Design

Designed by: Annakarin Korppoo   Group: iGEM15_Aalto-Helsinki   (2015-09-14)


Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2902
    Illegal BamHI site found at 573
    Illegal BamHI site found at 1271
    Illegal BamHI site found at 3791
    Illegal BamHI site found at 3929
    Illegal XhoI site found at 3690
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1379
    Illegal NgoMIV site found at 3608
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

The part sources are Haemophilus influenzae, Mycobacterium marinum (strain ATCC BAA-535 / M), and Bacillus subtilis for YciA, CAR and sfp, respectively. The promoter, RBS, and terminator sequences are derived from the commercial vector pCDF-Duet1 by Novagen.

References